Test kits

For rapid and efficient extraction of plasmid DNA from bacterial cells.The method is based on a combination of alkaline lysis and RNase treatment to obtain a clear lysate with minimal genomic DNA and RNA contamination. The bound plasmid DNA is washed and finally eluted by adding a buffer.SpecificationsMini Kit // Midi KitMethod:Spin column // Anion-ExchangeSample size:1 - 5 ml // 50 ml high-copy plasmid / 100 ml low-copy plasmidBinding capacity:30 µg // 800 µgFragment size:1 - 15 kb // 1 - 20 kbTypical yield:10- 30 µg // 200 - 800 µgOperation Time:15 min. // 120 min.

For rapid and efficient extraction of plasmid DNA from bacterial cells.The method is based on a combination of alkaline lysis and RNase treatment to obtain a clear lysate with minimal genomic DNA and RNA contamination. The bound plasmid DNA is washed and finally eluted by adding a buffer. The test kit contains the I-Blue Lysis Buffer, an optional colour indicator. The use of this indicator prevents common handling errors that impair efficient cell lysis and neutralization.Fast extraction timeHigh yieldPrevention of handling errors during cell lysis and neutralisation by I-Blue colour indicatorSpecificationsI-Blue Mini Plasmid Kit // I-Blue Midi Plasmid KitMethod:Spin column // Anion-ExchangeSample size:1 - 7 ml // 50 - 100 ml high-copy plasmid / 100 - 150 ml low-copy plasmidBinding capacity:50 µg // 500 µgFragment size:1 - 15 kb // 1 - 20 kbTypical yield:up to 50 µg // 200 - 500 µgOperation Time:15 min. // 80 min.

The test kit was developed to obtain or concentrate DNA fragments from agarose gels, PCR or other enzymatic processes.The agarose gel is dissolved, the enzymes are denatured and the DNA fragments will bind to the glass fibre matrix in the spin column. Wash buffers (containing ethanol) are used to remove contaminants and a low salt elution buffer is used to recover the purified DNA fragments. Recoveries are 90 - 95 % for PCR clean-up. With this kit, PCR purification and gel extraction procedures can be performed, so that a second test kit is not necessary.High recovery rateShort process timeOne test kit for two methodsSpecificationsSample size:up to 300 mg of agarose gel / up to 100 µl of PCR productBinding capacity:10 µg DNAFragment size:10 kbTypical yield:80-90% gel extraction / 90-95% PCR clean-upOperation Time:20 min.

Spin column system consisting of filter tubes with glass fibre filter and collection tubes. For the use of leftover reagents from IBI I-Blue MINI Plasmidpurification-Kits (IB47170, IB47171, IB47172), Gel / PCR / DNA Fragment Extraction Kits (IB47010, IB47020, IB47030), Plasmidpurification-Kits High-Speed MINI (IB47101, IB47102) or similar products from other suppliers using a binding, washing and elution method. Sample size, binding capacity and elution volume listed in the specifications must be observed.SpecificationsGel Fragment Extraction Kit // Plasmidpurification-KitsSample size:300 mg agarose gel / 100 µl PCR product // 1 - 4 ml bacterial cultureBinding capacity:up to 10 µg // up to 30 µgElution volume:20 - 50 µl // 50 - 100 µl

For the use of leftover reagents from IBI I-Blue MIDI Plasmid purification-Kits (IB47181), Plasmid purification-Kits MIDI Fast Ion (IB47111), Plasmid purification-Kits Maxi Fast Ion (IB47122), Plasmid purification-Kits Maxi Fast Ion, endotoxin-free (IB47125) or similar products from other suppliers using a binding, washing and elution method. Sample size and elution volume listed in the specifications must be observed.SpecificationsMIDI // MAXISample size:50 ml for high copy and 100 ml for low copy // 100 ml for high copy and 250 ml for low copyYield:up to 250 µg // up to 500 µgElution volume:8 ml // 12 mlOperation time:120 min. // 150 min.

For rapid and efficient extraction of plasmid DNA from bacterial cells. The method is based on a combination of alkaline lysis and RNase treatment to obtain a clear lysate with minimal genomic DNA and RNA contamination. The bound plasmid DNA is washed and finally eluted by adding a buffer.Fast extraction timeHigh yieldStandard version or endotoxin-free version (<0.10 EU/µg) availableSpecificationsMethod:Anion-ExchangeSample size:200 - 800 ml high-copy plasmid / 500 - 1600 ml low-copy plasmidBinding capacity:1.5 mgFragment size:1 - 20 kbTypical yield:1 - 1.5 mgOperation time:180 min.

Blue. FreezeBox™ is a new, energy-saving, environmentally friendly and safe cooling equipment, without additives. It does not require electricity (except for pre-cooling the cores in the freezer) and no ice for low-temperature cooling of biological samples. The system can be used ice-free, is lightweight, easy to transport and ideal for the collection and storage of biological samples. It can be combined with interchangeable cores and different tube modules. Cooling cores can be tempered in a refrigerator or freezer and inserted into the FreezeBox™. For operation with dry ice, leave out the cooling core and fill with 200 g dry ice instead.For use with cooling core, ice or dry iceEasy to clean and disinfectDurable, suitable for frequent useEasy to mount

The test kit was developed to obtain or concentrate DNA fragments from agarose gels, PCR or other enzymatic processes.The agarose gel is dissolved, the enzymes are denatured and the DNA fragments will bind to the glass fibre matrix in the spin column. Wash buffers (containing ethanol) are used to remove contaminants and a low salt elution buffer is used to recover the purified DNA fragments. Recoveries are up to 90 % for gel extraction and up to 95 % for PCR clean-up. With this kit, PCR purification and gel extraction procedures can be performed, so that a second test kit is not necessary.High recovery rateShort process timeOne test kit for two methodsSpecificationsSample size:up to 300 mg of agarose gel / up to 100 µl of PCR productElution volume:20-50 µlFragment size:50 bp - 200 bpTypical yield:90-95 % gel extraction and PCR clean-upOperation Time:20 min.

For use in any molecular biology application. Nuclease-free water is pure, quality-tested water suitable for use in all experiments that require nuclease-free water, including molecular biology applications. Nuclease-free water is prepared in a proprietary process, which yields DNase, RNase, and nuclease-free, deionized water without the use of chemical additives, such as diethylpyrocarbonate (DEPC). Nuclease-free water is provided in nuclease-free containers.

For 0.2 ml thermocycler blocksDetached cap strips can be used as individual tubes when cutWith domed or flat capsConsistent wall thickness for uniform heat transferContamination-free openingCap strips provide tight seal during all PCR cyclesVirgin polypropylene, certified RNase-, DNase- and Endotoxin-free

PCR plates with SafeCode use the three-level coding of the SafeCode system (datamatrix code, linear barcode, and clear writing) for reliable sample and tube identification in the laboratory. The codes are located at three sides of the plate. The ECC 200 error correction function enables safe code reading with up to 25 % damaged codes. ID-specific information on plates for documentation purposes is available via Eppendorf DataPort.Extremely thin-walled wells guarantee optimum temperature transferHigh-contrast labelling of wells for comfortable readingImport into database systems possibleCertified free of DNA, DNAase, RNase, and PCR inhibitorsManufactured from high-quality polypropylene

LLG-PCR tubes with flat caps are suitable for standard PCR/qPCR thermal cyclers. The uniform, thin tube wall ensures optimum heat transfer and minimises condensation. The ergonomic cap design makes it easy to open and close the tubes with one hand. The V shaped bottom allows total sample recovery. LLG-PCR tubes are autoclavable as well as RNase-, DNase-, and pyrogen-free. Withstands centrifugation up to 18000 x g in a fixed-angle rotor and up to 500 x g in a swing-out rotor.

LLG-PCR tubes with flat caps are suitable for standard PCR thermal cyclers. The uniform, thin tube wall ensures optimum heat transfer and minimises condensation. The V shaped bottom allows total sample recovery. LLG-PCR tubes are autoclavable as well as RNase-, DNase-, and pyrogen-free.

LLG-PCR tubes with flat, separate cap strips are suitable for standard PCR thermal cyclers. The uniform, thin tube wall ensures optimum heat transfer and minimises condensation. The V shaped bottom allows total sample recovery. LLG-PCR tubes are autoclavable as well as RNase-, DNase-, and pyrogen-free.

LLG-PCR plates are suitable for standard PCR thermocyclers. The PCR plates are equipped with a raised well rim, which ensures a secure seal with film, prevents evaporation, and minimises the risk of contamination. Each well is labelled with an alphanumeric code for easy sample identification. LLG-PCR plates are autoclavable as well as RNase-, DNase-, and pyrogen-free.

Peelable PE seals with strong, temperature-stable adhesive ensure ideal sealing of 96-well plates. Suitable for PCR applications. The seals withstand the thermal fluctuations of amplification cycles and prevent sample evaporation. LLG-PCR plate seals are RNase-, DNase-, and pyrogen-free.

Peelable aluminium seals with strong, temperature-stable adhesive ensure ideal sealing of 96-well plates. Suitable for PCR applications. The seals withstand the thermal fluctuations of amplification cycles and prevent sample evaporation. Easy to pierce. LLG-PCR plate seals are RNase-, DNase-, and pyrogen-free.

Good pierceabilityFor all plate types: PS, PP, PC, PEStorage in dry rooms at room temperature (approx. 20 to 23 °C)

Self-adhesive with high adhesive strengthHigh solvent resistanceNot pierceableFor manual use

Good pierceabilityDMSO-resistantNumbers indicate the upper side of the filmSealing temperature 160 to 180 °C, 2 to 3 sFor plate types: PS, PP

Optically clearPeelableNumbers indicate the top side of the filmSealing temperature 160 to 185 °C, 2 to 3 s